| Description |
1 online resource (1 page) : color illustrations. |
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text txt rdacontent |
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computer c rdamedia |
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online resource cr rdacarrier |
| Series |
NREL/PO ; 2700-82794 |
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NREL/PO ; 2700-82794.
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| Note |
"42nd Steenbock Symposium, June 7-8, 2022, Madison, Wisconsin." |
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In scope of the U.S. Government Publishing Office Cataloging and Indexing Program (C&I) and Federal Depository Library Program (FDLP). |
| Funding |
DE-AC36-08GO28308 |
| Note |
Description based on online resource; title from PDF title page (NREL, viewed October 21, 2022). |
| Summary |
A longstanding goal in the biofuel sector is to increase the efficiency of the circularization of the use of materials (1). One such instance is the degradation of plant material, such as cellulose, and converting it to biofuel via bacterial digestion (2). The bifunctional aldehyde-alcohol dehydrogenase (AdhE) from the anaerobic thermophile C. thermocellum seems to be vital for the production and cellular tolerance of bioethanol; however, it lacks the efficiency to produce ethanol at industry standards (3). Therefore, to understand how C. thermocellum AdhE functions, we used cryo-electron microscopy (cryo-EM) to obtain a 3.2 A structure of the AdhE complex. When compared to previously published structures from E. coli (4-6), we identified potential regions that control the native conformation of the ultrastructure, as well as located channels that isolate the intermediate aldehyde from the cellular milieu. This high-resolution structure, in combination with molecular dynamics simulations, provides insight into one example of substrate channeling and establishes a basis for future mutagenesis studies. |
| Subject |
Ethanol -- Synthesis.
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Bacteria.
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Biosynthesis.
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Éthanol -- Synthèse.
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Bactéries.
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Biosynthèse.
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Bacteria
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Biosynthesis
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| Indexed Term |
AdhE |
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bioethanol |
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C. thermocellum |
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cryo-electron microscopy |
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molecular dynamics |
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stimulations |
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structural biology |
| Added Author |
National Renewable Energy Laboratory (U.S.), issuing body.
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Center for Bioenergy Innovation (U.S.), sponsoring body.
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| Standard No. |
1873876 OSTI ID |
| Gpo Item No. |
0430-P-17 (online) |
| Sudoc No. |
E 9.28:NREL/PO-2700-82794 |
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