| Description |
1 online resource (160 pages) |
|
text txt rdacontent |
|
computer c rdamedia |
|
online resource cr rdacarrier |
| Note |
Print version record. |
| Contents |
Chapter 1 Why freeze human eggs? Clinical and biological indications for human oocyte cryopreservation; Ethical and legal indications; Fertility-saving indications; IVF laboratory flexibility indication; The controversial family planning indication; Chapter 2 The evolution of human oocyte cryopreservation; Preliminary research; Early animal studies; Early human studies; Subsequent research; Recent clinical application; Safety of oocyte cryopreservation; Chapter 3 Clinical management of oocyte cryopreservation; Ovarian stimulation and oocyte storage. |
|
Storage of surplus eggs in a standard cycleEgg storage to avoid ovarian hyperstimulation syndrome; Egg storage in polycystic ovary syndrome; Egg storage in cancer patients; Chapter 4 Laboratory management of oocyte cryopreservation; The egg freezing laboratory: location, equipment, instruments, materials; Selecting freezable eggs: the freezable egg score; Evaluation of oocyte maturity and quality; Evaluation of cumulus-corona-oocyte complex maturity; Methodology; Evaluation of the nucleus; Evaluation of the cytoplasm; Cytoplasmic abnormalities; Extracytoplasmic abnormalities. |
|
Zona pellucida (ZP)Perivitelline space (PVS); Perivitelline space granules; First polar body fragmentation; Evaluation of the meiotic spindle; Grading of the oocyte; The freezable egg score; Survived oocyte; Degenerated first polar body; Degenerated oocyte; Oocyte with some sign of degeneration; Steps and timing of the entire cryopreservation procedure; Timing of the events that occur after HCG administration until embryo transfer; Timing of semen sample production; Organizing criteria for storage in liquid nitrogen tanks; Cryobiology; Chapter 5 Slow freezing; Principles. |
|
Evolution of the slow freezing methodologySlow freezing/rapid thawing; Materials; Solutions for slow freezing; Thawing solutions for human oocytes; Setting up for a freezing cycle; Setting up for a thawing cycle; Freezing solutions at different sucrose concentrations; Thawing solutions with differentiated sucrose concentrations; Setting up for a freezing cycle with differential sucrose concentrations; Setting up for a thawing cycle with differential sucrose concentrations; Recipe formula for Na depleted PBS. |
|
Volume, temperature and time of exposure during slow freezing utilizing solutions with 0.1 or 0.2 or 0.3M sucrose at freezing and thawing (freezing 0.1M/thawing 0.1M-freezing 0.2M/thawing 0.2M-freezing 0.3M/thawing 0.3M)Volume, temperature and time of exposure during slow freezing utilizing solutions with differentiated sucrose concentrations (freezing 0.2M sucrose/thawing 0.3M); Starting the freezing cycle; Loading the straw into the programmable freezer; Beginning of the freezing program; Seeding; Storage of the samples; Automated computerized freezing machine; Rapid thawing; Methodology. |
| Note |
Volume, temperature and time of exposure during rapid thawing. |
| Summary |
An indispensable practical tool for embryologists and reproductive medicine specialists seeking to offer their patients this revolutionary treatment option. |
| Bibliography |
Includes bibliographical references and index. |
| Subject |
Cryopreservation of organs, tissues, etc.
|
|
Oocytin -- Cytology.
|
|
Cryopreservation -- methods |
|
Oocytes -- cytology |
|
Cryopreservation |
|
Cryoconservation des organes, tissus, etc.
|
|
NATURE -- Reference.
|
|
SCIENCE -- Life Sciences -- Biology.
|
|
SCIENCE -- Life Sciences -- General.
|
|
Cryopreservation of organs, tissues, etc.
|
| Genre/Form |
Methods (Music)
|
| Added Author |
Ciotti, Patrizia.
|
|
Venturoli, Stefano.
|
| Other Form: |
Print version: Porcu, Eleonora. Handbook of Human Oocyte Cryopreservation. Cambridge : Cambridge University Press, ©2012 9780521192392 |
| ISBN |
9781139841955 |
|
1139841955 |
|
9781139839570 (electronic bk.) |
|
1139839578 (electronic bk.) |
|
9780511977879 (electronic bk.) |
|
0511977875 (electronic bk.) |
|
9780521192392 |
|
0521192390 |
| Standard No. |
DEBSZ 397421419 |
|
DEBSZ 431258503 |
|
