| Description |
1 online resource |
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text txt rdacontent |
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computer c rdamedia |
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online resource cr rdacarrier |
| Series |
Wilson and Wilson's comprehensive analytical chemistry ; volume 64 |
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Wilson and Wilson's comprehensive analytical chemistry ; v. 64.
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| Bibliography |
Includes bibliographical references and index. |
| Note |
Online resource; title from PDF title page (Ebsco; viewed on May 16, 2014). |
| Summary |
The book contains contributions concerning the application of the new instrumental and methodological developments in omics technologies, including those related to Genomics, Transcriptomics, Proteomics, Peptidomics and Metabolomics, Lipidomics and Foodomics. The16 chapters discuss in detail: innovative applications of functional gene microarrays for profiling microbial communities, microRNA profiling, novel genotyping applications using microarray technology in cancer research, next-generation sequencing applied to the study of human microbiome, emerging RNA-SEQ applications in food science, recent progress in plant proteomics, applications of gel-free proteomic approaches, the challenges and applications of proteomics tools for food authenticity, the role of salivary peptidomics in clinical applications, metabolomic approaches to the study of degenerative, cardiovascular and renal diseases, and neonatal medicine. Also covered are other omics applications such as profiling of genetically modified organisms, the fundamentals, applications and challenges of foodomics, and MS-based lipidomics. Moreover, this volume includes relevant and updated aspects on bioinformatics, data treatment, data integration and systems biology. This book complements the previous volume "Fundamentals of Advanced Omics Technologies: New Advances from Genes to Metabolites" that covered the fundamental aspects of these new omics technologies |
| Contents |
Front Cover; Applications of Advanced Omics Technologies: From Genes to Metabolites; Copyright; Dedication; Contents; Contributors to Volume 64; Series Editor Preface; Preface; Part I: Genomics and Transcriptomics; Chapter 1: Applications of Functional Gene Microarrays for Profiling Microbial Communities; 1. Introduction; 2. Comparison of Microarrays with High-Throughput Sequencing; 3. Functional Gene Arrays; 3.1. GeoChip Microarrays; 3.2. Other FGAs; 3.2.1. Hydrogenase Chip; 3.2.2. Food Safety and Health Applications; 3.2.3. Detection of Novel Sequences. |
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3.2.4. Other Microarrays: Unique Applications4. Key Issues in Microarray Application; 5. Future Advancements; 6. Conclusions; Acknowledgments; References; Chapter 2: microRNA Profiling: An Overview of Current Technologies and Applications; 1. Introduction; 2. miRNA Biogenesis and Nomenclature; 3. Considerations for miRNA Profiling; 4. miRNA Extraction; 5. qPCR Analysis of miRNAs; 6. Microarray Profiling of miRNAs; 7. Small RNA-seq; 8. In Silico miRNA Analysis Resources; 9. miRNA Functional Analysis; 10. Recent Developments in the Application of miRNA Profiling to Cancer Research. |
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11. ConclusionsReferences; Chapter 3: Application of Novel Genotyping Microarray Technologies in Cancer Research; 1. Introduction; 2. Cancer Genetics: From Biology to Clinical Practice; 2.1. The Discovery of Cancer Genetics; 2.2. The Introduction of Cancer Genetics on Cancer Therapy; 2.3. Tumor Genome Analysis Applied to Improve Cancer Therapy; 3. Genotyping Technologies; 3.1. The Study of Defined Variants at the Level of DNA; 3.1.1. Variants Discovery by Sequencing; 3.1.2. Alternative Techniques for the Analysis of Defined Variants; 3.1.3. Real-Time PCR-Based Techniques. |
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3.1.4. Whole-Genome SNP Analysis3.2. Analysis of DNA Variation Independent from Single Nucleotide Polymorphisms; 3.3. Variants Found in Small Numbers; 3.4. Gene Expression Studies; 4. Next-Generation Genomic Techniques; 5. Applications of Novel SNP Technologies in Cancer Research; 6. Conclusions and Future Directions; References; Chapter 4: Applications of Next-Generation Sequencing Technologies to the Study of the Human Microbiome; 1. Introduction; 1.1. 16S Sequencing Technologies; 2. Next-Generation Sequencing; 2.1. Roche 454 Pyrosequencing; 2.1.1. Barcoding; 2.1.2. Sequencing Parameters. |
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2.2. Illumina SBS Platform (Genome Analyzer IIx, HiSeq, MiSeq)2.3. AB (Applied Biosystems) SOLiD Platform (AB 5500, 5500xl); 2.4. Pacific Bioscience SMRT Platform (PacBio RS II); 2.5. Ion Torrent (PostLightTM) Semiconductor Platform (Ion PGM, Ion Proton); 3. Bioinformatics; 3.1. Denoising; 3.2. Detecting Chimeras; 3.3. Operational Taxonomic Units; 3.4. Sequence Alignment; 3.5. Phylogenetic Analysis; 3.6. Estimating Diversity; 3.7. Comparing Between Communities; 3.8. Unifying Platforms; 4. The Microbiome in Health and Disease; 4.1. The Gastric Microbiome. |
| Subject |
Molecular biology -- Technological innovations.
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Bioinformatics.
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Computational Biology |
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Biologie moléculaire -- Innovations.
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Bio-informatique.
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SCIENCE -- Life Sciences -- Biochemistry.
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Bioinformatics
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| Added Author |
García-Cañas, Virginia, editor.
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Cifeutes, Alejandro, editor.
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Simó, Carolina, editor.
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| ISBN |
9780444626691 (electronic bk.) |
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0444626697 (electronic bk.) |
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9780444626509 |
| Standard No. |
AU@ 000062545376 |
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DEBSZ 414275608 |
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DEBSZ 431685002 |
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