| Description |
1 online resource (45 pages) : color illustrations. |
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text txt rdacontent |
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computer c rdamedia |
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online resource cr rdacarrier |
| Series |
NREL/PR ; 2700-79338 |
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NREL/PR ; 2700-79338.
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| Note |
"Biochemical Conversion and CO2Utilization." |
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"March 8, 2021." |
| Bibliography |
Includes bibliographical references (pages 41-43). |
| Funding |
National Renewable Energy Laboratory DE-AC36-08GO28308 |
| Note |
Description based on online resource; title from PDF title page (NREL, viewed on Oct. 22, 2021). |
| Summary |
The FY2020 SOT economic model for the DMR BDO process indicates that the production and use of biomass degrading enzymes represents ~10% of the MFSP of BDO. We are thus working to enhance the performance of the dominant cellulase enzyme, Cel7A. In Fy2018-19, we selected 100 promising genes from the "Cel7A wheel of life" and cloned them into T. reesei using a constitutive promotor. This natural diversity screening resulted in the discovery of several enzymes exhibiting improved characteristics relative to the industry standard Cel7A from T. reesei (Tr). Growth of the transformed host on glucose insured that the Cre1 induced cellulase expression cascade was suppressed, thus enabling purification of the target gene product. The first top performing enzyme found was from P. funiculosum (Pf). Other top performing enzymes were identified from T. aculeatus, T. terrestris, and A. oryzae. Computational modeling predicted the structural subsites responsible for the improvements, which were cloned into the sequence of PfCel7A. The recombinant enzymes were expressed in T. reesei, purified, and tested. Testing was not possible with SOT relevant substrates, so the best-case substrate was used; solids from the DMR process subjected to a novel dilute alkaline wash to remove the "lignin shield." Two chimeric Cel7A enzymes show improved performance (1.2 to 1.35x) relative to PfCel7A, which in turn showed considerable improvement (1.6x) relative to T. reesei Cel7A. These new enzymes have been provided to our industrial partner for evaluation. |
| Subject |
United States. Department of Energy. Bioenergy Technologies Office.
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Enzymes -- Biotechnology -- United States.
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Biotechnology -- Environmental aspects -- United States.
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Peer review -- United States.
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Enzymes -- Biotechnologie -- États-Unis.
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Évaluation par des pairs -- États-Unis.
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Biotechnology -- Environmental aspects
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Enzymes -- Biotechnology
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Peer review
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United States https://id.oclc.org/worldcat/entity/E39PBJtxgQXMWqmjMjjwXRHgrq
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| Indexed Term |
biochemical conversion |
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CO2 utilization |
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enzyme loading |
| Genre/Form |
Congress |
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technical reports.
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proceedings (reports)
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Technical reports
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Conference papers and proceedings
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Technical reports.
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Conference papers and proceedings.
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Rapports techniques.
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Actes de congrès.
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| Added Author |
National Renewable Energy Laboratory (U.S.), issuing body.
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| Added Title |
Bioenergy Technologies Office 2021 peer review : enzyme engineering and optimization (EEO) |
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Enzyme engineering and optimization (EEO) |
| Standard No. |
1772966 OSTI ID |
| Gpo Item No. |
0430-P-09 (online) |
| Sudoc No. |
E 9.22:NREL/PR-2700-79338 |
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