Front Cover; Peptides and Proteins as Biomaterials for Tissue Regeneration and Repair; Copyright; Contents; Contributors; Preface; Peptides and proteins as biomaterials for tissue regeneration and repair; Chapter 1: Fundamentals of protein and cell interactions in biomaterials; 1.1 Fundamentals of protein adsorption on biomaterials; 1.1.1 Basics of protein adsorption; 1.1.1.1 Function and structural organization; 1.1.1.2 Structure and orientation of adsorbed proteins; 1.1.2 Interactions with the surface: hydrophobic and electrostatic bonding; 1.1.3 Kinetics of protein adsorption.
1.1.4 Conformational changes and stability1.1.5 Reversibility of protein adsorption; 1.1.6 Competitive adsorption behavior; 1.2 Biomaterial surface properties and their effect on protein adsorption; 1.2.1 Promoting protein adsorption: Osseointegration; 1.2.2 Preventing protein adsorption: Hemocompatibility; 1.3 Quantification of protein adsorption; 1.3.1 Optical; 1.3.1.1 Ellipsometry; 1.3.1.2 Surface plasmon resonance; 1.3.2 Spectroscopic; 1.3.2.1 Fluorescent spectroscopy; 1.3.2.2 Infrared absorption spectroscopy; 1.3.3 Microscopic; 1.3.3.1 Atomic force microscopy (AFM); 1.3.4 Radiolabeling.
1.3.5 Quartz crystal microbalance with dissipation monitoring (QCM-D)1.4 The importance of adsorbed proteins in the tissue reaction to biomaterials; 1.4.1 Effect of adsorbed proteins on cell adhesion; 1.4.2 Effect of adsorbed proteins on cell activation; 1.4.3 Effect of adsorbed proteins on the FBR; 1.5 Quantification/detection of cell adhesion and activation; 1.5.1 Cell adhesion; 1.5.1.1 Micropatterning; 1.5.1.2 Three-dimensional traction force microscopy (3D-TFM); 1.5.1.3 QCM-D; 1.5.1.4 Microfluidic; 1.5.1.5 AFM; 1.5.2 Cell activation; 1.5.2.1 Fluorescence microscopy; 1.5.2.2 Flow cytometry.
1.5.2.3 Enzyme-linked immunosorbent assay (ELISA)1.6 Concluding remarks; References; Chapter 2: Extracellular matrix constitution and function for tissue regeneration and repair; 2.1 An overview of ECM structure and function; 2.1.1 Architectural role; 2.1.2 Adhesion mediator; 2.1.3 Mechanosensor; 2.1.4 Growth factor reservoir and modulator of signaling peptides; 2.2 Major ECM components; 2.2.1 Collagen; 2.2.2 Proteoglycans; 2.2.3 Other ECM molecules; 2.2.4 Matrix-degrading enzymes; 2.3 ECM dynamics in development; 2.3.1 General aspects/processes; 2.3.1.1 Embryogenesis.
2.3.1.2 Branching morphogenesis2.3.1.3 Stem cell niches and stem cell differentiation; 2.3.1.4 Homeostasis; 2.3.2 How systems work; 2.3.2.1 ECM in nervous system development; 2.3.2.2 Skeletal development; 2.3.2.3 Skin development; 2.4 ECM remodeling in regeneration and repair; 2.4.1 Intervertebral disc regeneration; 2.4.2 Wound healing; 2.4.3 Bone remodeling and healing; 2.4.4 CNS regeneration and repair; 2.5 Conclusions; References; Chapter 3: Surface functionalization of biomaterials for bone tissue regeneration and repair; 3.1 General introduction and chapter overview.
