| Description |
1 online resource |
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text txt rdacontent |
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computer c rdamedia |
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online resource cr rdacarrier |
| Summary |
Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. |
| Contents |
Front cover -- Half title -- Full title -- Copyright -- Contents -- Preface -- Chapter 1 -- Fundamentals of imaging mass spectrometry -- Principles of imaging mass spectrometry -- Advantages and limitation of imaging mass spectrometry -- References -- Chapter 2 -- Ionization sources for imaging mass spectrometry -- Matrix-assisted laser desorption/ionization -- Desorption electrospray ionization -- MALDI-2 -- Laser ablation electrospray ionization -- Secondary ion MS -- Laser ablation inductively coupled plasma -- Liquid extraction surface analysis -- Nanospray desorption electrospray ionization -- Conclusions -- References -- Chapter 3 -- Sample preparation for imaging mass spectrometry -- Sectioning and storage -- Mounting -- Imprinting and stamping -- Tissue storage -- Tissue drying -- Tissue rinsing and incubation -- Tissue washing -- Deparaffinization -- Pre-extraction -- Other tissue incubation procedures -- On-tissue chemistry -- On-tissue chemical derivatization -- Antigen retrieval -- Immunohistochemistry -- On-tissue in situ enzymatic digestion -- Heat stabilization of tissue -- In-plume microdroplet reactions -- Adding chemicals to tissue -- Chemical doping -- Matrix for MALDI -- Conclusions -- References -- Chapter 4 -- Tissue sectioning for imaging mass spectrometry -- Tissue collection -- Snap freezing -- Fixing or embedding -- Tissue orientation -- Cryosectioning -- Mounting -- Tissue section storage -- Conclusions -- References -- Chapter 5 -- Matrix for matrix-assisted laser desorption/ionization (MALDI) -- Matrix application -- Manual matrix application by airbrushing -- Automated matrix deposition -- Application of matrix by sublimation -- Precoated matrix slides -- Dry-coating matrix -- Types of matrices for MALDI -- Nanomaterial as a matrix -- Reactive matrix -- Matrix selection -- Matrix stability -- Conclusions. |
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Chapter 11 -- Multimodal imaging mass spectrometry -- Image registration -- Multimodal imaging techniques in imaging MS -- Multimodal imaging MS with multiple ion sources -- Multimodal imaging MS with optical microscopy -- Multimodal imaging MS with clinical imaging -- Multimodal imaging MS with other imaging techniques -- Conclusions -- References -- Chapter 12 -- Validation and standardization of imaging mass spectrometry -- Validation of imaging MS -- Specificity -- Robustness -- Sensitivity -- Linearity and dynamic range -- Stability -- Accuracy and imprecision -- Postvalidation -- Standardization of imaging MS -- Standardization -- Standardized data format-imzML -- Reporting standards for imaging MS -- Multicenter validation of imaging MS -- Conclusions -- References -- Chapter 13 -- Toward clinical imaging mass spectrometry -- Biomedical visualization in clinic -- Histopathology -- Digital pathology -- In vivo imaging -- Labeling in clinical imaging -- Clinical application of imaging MS -- Pathology -- Intraoperative histopathology -- Tissue microarrays -- Treatment response -- Clinical implementation of imaging MS -- Medical device -- Laboratory-developed test -- Outlook -- References -- Chapter 14 -- Imaging mass spectrometry: endogenous mammalian metabolites -- Improved sample preparation for metabolite imaging -- Enhancing metabolite detection in MALDI by matrix selection -- Increased mass resolution for metabolite imaging -- Imaging metabolite with ion mobility separation MS -- Using multiple ion sources for metabolite imaging MS -- Outlook -- References -- Chapter 15 -- Imaging mass spectrometry: Glycans -- MALDI imaging of glycans -- Targeted glycan imaging -- Outlook -- References -- Chapter 16 -- Imaging mass spectrometry: steroids mapping using on-tissue chemical derivatization -- On-tissue chemical derivatization workflow. |
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On-tissue chemical derivatization and imaging of steroids -- Outlook -- References -- Chapter 17 -- Imaging mass spectrometry: neurotransmitter distribution using reactive matrix and chemical derivatization -- Reactive matrix for imaging neurotransmitters -- On-tissue chemical derivatization for imaging neurotransmitters -- Outlook -- References -- Chapter 18 -- Imaging mass spectrometry: small drugs and metabolites in tissue -- Increasing sensitivity for drug imaging by MS -- Increasing selectivity for drug imaging MS -- Outlook -- References -- Chapter 19 -- Imaging mass spectrometry: gangliosides in brain tissue -- Matrix selection for ganglioside for MALDI imaging -- Atmospheric pressure MALDI for ganglioside imaging -- Sample preparation for enhanced ganglioside imaging by MS -- Novel developments in ganglioside imaging by MS -- Outlook -- References -- Index -- Back cover. |
| Subject |
Biomolecules -- Analysis.
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Mass spectrometry -- Methodology.
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Biomolécules -- Analyse.
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Spectrométrie de masse -- Méthodologie.
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Biomolecules -- Analysis
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| Other Form: |
Print version: 0128189983 9780128189986 (OCoLC)1150961151 |
| ISBN |
9780128189993 (electronic bk.) |
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0128189991 (electronic bk.) |
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9780128189986 |
| Standard No. |
AU@ 000069149098 |
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AU@ 000069303299 |
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UKMGB 020135611 |
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