| Edition |
2nd ed. |
| Description |
xviii, 252 p. : ill. ; 24 cm. |
| Series |
The Practical approach series ; 138
|
| Bibliography |
Includes bibliographical references and index. |
| Contents |
Plasmid replication and maintenance : Introduction --Single-cell resistance (SCR) to antibodiotics -- Determination of plasmid copy numbers -- Partition and other maintenance functions --Incompatibility -- Basic replicons -- Origins (ori) of replication -- Rep functions -- Cop functions -- Replicative intermediates -- Timing of replication -- In vitro replication -- Visualization of plasmids / Kurt Nordstrom -- Plasmids from gram-positive bacteria : Introduction --Classification -- Isolation of plasmid DNA - Host range and plasmid transfer -- Plasmid replication modes / Juan C. Alonso and Manuel Espinosa -- Lactococcal plasmid vectors : Introduction -- Lactococcal vector systems / K. J. Leenhouts and G. Venema. |
|
Virulence plasmids : Introduction -- Isolation of plasmids --Curing of plasmids -- Mutagenesis -- Fast and small electrophoresis sequencing gels-- Comparison of helical stability of DNA fragments --Identification of plsamid-encoded products -- RNA extraction and analysis / Marcelo E. Tolmasky, Luis A. Actis, and Jorge H. Crosa -- Plasmids in Agrobacterium tumefaciens and Rhodococcus fascians : Introduction --Identification of endogenous plasmids by small scale isolation procedures -- Large scale purification methods -- Introduction of recombinant plasmids -- Analysis of plasmid-encoded functions / Jan Desomer, Rolf Deblaere, and Marc Van Montagu -- Streptomyces plasmid vectors : Introduction -- Autonomously replicating Streptomyces vectors --Integrating vectors -- Gene disruption/replacement techniques using temperature-sensitive and non-replicative plasmids / W. Wohlleben and G. Muth. |
|
Use of [Lambda]-plasmid composite vectors for expression cDNA cloning : Introduction -- Expression in cloning using unidrectional cDNA libraries -- Generation of cDNA libraries by the ADC method -- Analysis of cloned cDNAs -- Applications / Toru Miki and Stuart A. Aaronson --Phagemids and other hybrid vectors : Introduction -- Early Vectors -- New applications -- Phagemids and lambda ZAP cloning -- Future / Michelle A. Alting-Mees, Peter Vaillancourt, and Jay M. Short -- Extrachromosomal cloning vectors of Saccharomyces cerevisiae : Introduction -- Basic cloning vectors of S. cerevisiae -- Multifunctional cloning vectors of S. cerevisiae -- Yeast host strain -- Introduction of DNA into S. cerevisiae / Robert S. Sikorski. |
| Subject |
Plasmids -- Research -- Methodology.
|
| Added Author |
Hardy, K. G. (Kimber G.)
|
| ISBN |
0199634440 (pbk.) : $30.00 |
|
0199634459 (hbk.) : $45.00 |
|
