| Description |
1 online resource (xiv, 722 pages) : illustrations |
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text txt rdacontent |
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computer c rdamedia |
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online resource cr rdacarrier |
| Series |
Separation science and technology ; v. 2 |
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Separation science and technology (San Diego, Calif.) ; v. 2.
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| Bibliography |
Includes bibliographical references and index. |
| Contents |
Bioseparations : an overview / S. Ahuja -- Analysis of protein impurities in pharmaceuticals derived from recombinant DNA / Donald O. O'Keefe -- Physicochemical factors in polypeptide and protein purification and analysis by high-performance liquid chromatographic techniques : current status and challenges for the future / Milton T.W. Hearn -- Capillary electrophoresis of compounds of biological interest / S. Ahuja -- Isoelectric focusing / David E. Garfin -- Mass spectrometry of biomolecules / Dan Gibson and Catherine E. Costello -- Liquid-liquid partitioning methods for bioseparations / Tingyue Gu -- Separation of nucleic acids and proteins / Rohit Harve and Rakesh Bajpai -- Bioseparations by displacement chromatography / Steven M. Cramer -- Physicochemical basis of expanded-bed adsorption for protein purification / B. Mattiasson and M.P. Nandakumar -- Expanded-bed adsorption process for protein capture / Joseph Shiloach and Robert M. Kennedy -- Adsorptive membranes for bioseparations / Ranjit R. Deshmukh and Timothy N. Warner -- Simulated moving-bed chromatography for biomolecules / R.M. Nicoud -- Large-scale chromatographic purification of ougonucleotides / Ranjit R. Deshmukh, William E. Leitch II, Yogesh S. Sanghvi and Douglas L. Cole -- Separation of antibodies by liquid chromatography / Egisto Boschetti and Alois Jungbauer -- Processing plants and equipment / P. Bowles -- Engineering process control of bioseparation processes / Randel M. Price and Ajit Sadana -- Economics of bioseparation processes / Anand Ramakrishnan and Ajit Sadana -- Future developments / S. Ahuja. |
| Summary |
It is generally recognized that the commercial success of biotechnology products is highly dependent on the successful development and application of high-powered separation and purification methods. In this practical and authoritative handbook, the separation of proteins, nucleic acids, and oligonucleotides from biological matrices is covered from analytical to process scales. Also included in a chapter on the separation of monoclonal antibodies, which have found numerous uses as therapeutic and diagnostic agents. Analytical techniques include an interesting montage of chromatographic methods, capillary electrophoresis, isoelectric focusing, and mass spectrometry. Among separation and purification methods, liquid-liquid distribution, displacement chromatography, expanded bed adsorption, membrane chromatography, and simulated moving bed chromatography are covered at length. Regulatory and economic considerations are addressed, as are plant and process equipment and engineering process control. A chapter on future developments highlights the application of DNA chip arrays as well as evolving methodologies for a large number of drugs that are under development for treatment of cancer, AIDS, rheumatoid arthritis, and Alzheimer's disease. Handbook of Bioseparations serves as an essential reference and guidebook for separation scientists working in the pharmaceutical and biotechnology industries, academia, and government laboratories. Key Features * Covers bioseparations of proteins, nucleic acids, and monoclonal antibodies * Encompasses both analytical and process-scale methods * Elucidates the importance of engineering process control * Details selection of plant and process equipment * Addresses economic considerations * Discusses future developments. |
| Note |
Print version record. |
| Subject |
Biomolecules -- Separation -- Handbooks, manuals, etc.
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Biomolecules -- Separation.
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Proteins -- isolation & purification |
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Chromatography -- methods |
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Mass Spectrometry -- methods |
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Biomolécules -- Séparation.
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Biomolécules -- Séparation -- Guides, manuels, etc.
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SCIENCE -- Life Sciences -- Molecular Biology.
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Biomolecules -- Separation
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| Genre/Form |
Handbook |
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handbooks.
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Handbooks and manuals
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Handbooks and manuals.
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Guides et manuels.
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| Added Author |
Ahuja, Satinder, 1933-
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| Other Form: |
Print version: Handbook of bioseparations. San Diego, CA : Academic Press, 2000 0120455404 9780120455409 (DLC) 00102786 (OCoLC)44058424 |
| ISBN |
9780120455409 |
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0120455404 |
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9780080507798 |
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0080507794 |
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1281058890 |
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9781281058898 |
| Standard No. |
AU@ 000051860035 |
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AU@ 000056736146 |
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DEBBG BV039832650 |
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DEBBG BV042308285 |
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DEBSZ 316158011 |
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DEBSZ 482436123 |
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NZ1 12433986 |
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