Advances in planar lipid bilayers and liposomes. Volume twenty one / edited by Professor Dr. Ale Igli, Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia [and four others].
Online resource; title from PDF title page (ebrary, viewed April 2, 2015).
Contents
Front Cover; Advances in Planar Lipid Bilayers and Liposomes; Copyright; Contents; Contributors; Preface; Chapter One: Development of Polymer/Nanodiamond Composite Coatings to Control Cell Adhesion, Growth, and Functions; 1. Introduction; 2. Materials and Methods; 2.1. Synthesis of polymer/nanodiamond composite layers; 2.2. Surface characterization of the composite layers; 2.3. Cell culture experiments; 2.3.1. Cell cultures; 2.3.2. Cell adhesion assay; 2.3.3. Scanning electron microscopy; 2.3.4. Immunofluorescent labeling of the actin cytoskeleton; 2.3.5. Cell proliferation assay
2.3.6. ALP activity assay2.4. Statistical analysis; 3. Results; 3.1. Characterization of the composite layers; 3.2. Cell adhesion; 3.3. Organization of the actin cytoskeleton; 3.4. Cell proliferation; 3.5. ALP activity; 4. Discussion; 5. Conclusions; Acknowledgments; References; Chapter Two: Tethered Phospholipid Bilayer Membranes: An Interpretation of the Electrochemical Impedance Response; 1. Introduction; 2. Electrochemical Impedance Spectroscopy; 2.1. Definition; 2.2. Equivalent circuit models of impedance; 2.3. Electrochemical impedance of defect-free tBLMs
3. Electrochemical Impedance Response of Defects3.1. Phenomenological background for EI model; 3.2. Impedance of submembrane reservoir; 3.3. Total impedance of tBLMs containing defects; 4. tBLM Parameters That Determine EI Response; 4.1. Density of defects; 4.2. Specific resistance of the submembrane reservoir; 4.3. Helmholtz capacitance; 4.4. Phospholipid bilayer capacitance; 4.5. Clustering of defects; 5. Conclusions; Acknowledgments; References; Chapter Three: Microscopy of Model Membranes: Understanding How Bcl-2 Proteins Mediate Apoptosis; 1. Introduction
2. Model Membranes and Techniques in Studying Bcl-2 Proteins2.1. Lipids of the mitochondria; 2.2. Supported lipid bilayers; 2.3. Liposomes; 2.3.1. Large unilamellar vesicles; 2.3.2. Giant unilamellar vesicles; 2.4. Techniques to study model membranes and membrane proteins; 2.4.1. Atomic force microscopy; 2.4.2. Fluorescence-based assays and fluorescence microscopy; 2.4.3. Fluorescence correlation spectroscopy; 2.4.3.1. Scanning fluorescence correlation spectroscopy; 2.4.3.2. Two-color fluorescence cross-correlation spectroscopy; 3. Uncovering the Mechanisms of the Bcl-2 Family
3.1. Mechanistic differences in membrane permeabilization of Bcl-2 family members: Bax versus Bcl-xL3.2. Bax and Bak form pores of tunable size; 3.3. Peptides of Bax mimic its pore-forming activity by decreasing line tension in membranes; 3.4. Role of membranes in promoting interaction between Bcl-2 family members; 4. Summary and Future Directions; References; Chapter Four: Optical Microscopy of Giant Vesicles as a Tool to Reveal the Mechanism of Action of Antimicrobial Peptides and ... ; 1. Introduction; 2. Experimental Approaches Based on Optical Microscopy of GUVs
