| Description |
1 online resource (xix, 672 pages) : illustrations |
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text txt rdacontent |
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computer c rdamedia |
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online resource cr rdacarrier |
| Series |
Journal of chromatography library ; v. 58 |
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Journal of chromatography library ; v. 58.
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| Summary |
Carbohydrates and glycoconjugates play an important role in several life processes. The wide variety of carbohydrate species and their inherent polydispersity and heterogeneity require separation techniques of high resolving power and high selectivity such as high performance liquid chromatography (HPLC) and capillary electrophoresis (HPCE). In the last decade HPLC, and recently HPCE methods have been developed for the high resolution and reproducible quantitation of carbohydrates. Despite the importance of these two column separation technologies in the area of carbohydrates, no previous book describes specialized methods for the separation, purification and detection of carbohydrates and glycoconjugates by HPLC and HPCE. Therefore, the objective of the present book is to provide a comprehensive review of carbohydrate analysis by HPLC and HPCE by covering analytical and preparative separation techniques for all classes of carbohydrates including mono- and disaccharides; linear and cyclic oligosaccharides; branched heterooligosaccharides (e.g., glycans, plant-derived oligosaccharides); glycoconjugates (e.g., glycolipids, glycoproteins); carbohydrates in food and beverage; compositional carbohydrates of polysaccharides; carbohydrates in biomass degradation; etc. The book will be of interest to a wide audience, including analytical chemists and biochemists, carbohydrate, glycoprotein and glycolipid chemists, molecular biologists, biotechnologists, etc. It will also be a useful reference work for both the experienced analyst and the newcomer as well as for users of HPLC and HPCE, graduates and postdoctoral students. |
| Bibliography |
Includes bibliographical references and index. |
| Note |
Print version record. |
| Contents |
Front Cover; CarbohydrateIm Analysis: High Performance Liquid Chromatography and Capillary Electrophoresis; Copyright Page; Contents; Preface; List of Contributors; Part I: The Solute; Chapter 1. Preparation of Carbohydrates for Analysis by HPLC and HPCE; 1.1 Introduction; 1.2 Sample purification; 1.3 Release of poly- or oligosaccharides from the sample; 1.4 Further degradation for structural study; 1.5 Exchanging solvents; 1.6 Sources for reference compounds; 1.7 References; Part II: Analytical and Preparative Separations |
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Chapter 2. Reversed-Phase and Hydrophobic Interaction Chromatography of Carbohydrates and Glycoconjugates2.1 Introduction; 2.2 Fundamentals; 2.3 Stationary phases; 2.4 Mobile phases; 2.5 Separation methodologies and selected applications; 2.5 Acknowledgments; 2.6 References; Chapter 3. High Performance Hydrophilic Interaction Chromatography of Carbohydrates with Polar Sorbents; 3.1 Introduction; 3.2 The chromatographic system; 3.3 Applications of hydrophilic interaction chromatography to carbohydrates; 3.4 Examples of applications of hydrophilic interaction chromatography to carbohy- drates |
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3.5 Conclusions3.6 References; Chapter 4. HPLC of Carbohydrates with Cation- and Anion-Exchange Silica and Resin-Based Stationary Phases; 4.1 Introduction; 4.2 Cation-exchange HPLC of carbohydrates; 4.3 Anion-exchange HPLC of carbohydrates; 4.4 Applications; 4.5 References; Chapter 5. Analysis of Glycoconjugates Using High-pH Anion-Exchange Chromatography; 5.1 Introduction; 5.2 Monosaccharide analysis; 5.3 Oligosaccharide analysis; 5.4 In-line and off-line desalting after HPAEC separations; 5.5 Other important applications; 5.6 Abbreviations; 5.7 References |
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Chapter 6. Basic Studies on Carbohydrate -- Protein Interaction by High Performance Affinity Chromatography and High Performance Capillary Affinity Electrophoresis Using Lectins as Protein Models6.1 Introduction; 6.2 Use of high performance liquid chromatography; 6.3 Use of high performance capillary electrophoresis; 6.4 References; Chapter 7. Modern Size-Exclusion Chromatography of Carbohydrates and Glycoconjugates; 7.1 Introduction; 7.2 The chromatographic system; 7.3 Applications of size-exclusion chromatography in the carbohydrate field; 7.4 Conclusions; 7.5 References |
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Chapter 8. High Performance Capillary Electrophoresis of Carbohydrates and Glycoconjugates8.1 Introduction; 8.2 Overview of the fundamentals of capillary electrophoresis; 8.3 The electrophoretic system; 8.4 HPCE methodologies and applications; 8.5 Acknowledgments; 8.6 References; Chapter 9. Preparative HPLC of Carbohydrates; 9.1 Introduction; 9.2 Equipment; 9.3 Stationary phases; 9.4 General guidelines for preparative HPLC of carbohydrates; 9.5 Specific preparative HPLC techniques for various classes of carbohydrates; 9.6 Bibliographic information; 9.7 References; Part III: The Detection |
| Language |
English. |
| Subject |
Carbohydrates -- Analysis.
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High performance liquid chromatography.
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Capillary electrophoresis.
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Carbohydrates -- analysis |
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Chromatography, High Pressure Liquid |
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Glycoconjugates -- analysis |
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Chromatography, High Pressure Liquid -- methods |
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Electrophoresis -- methods |
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Glucides -- Analyse.
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Chromatographie en phase liquide à hautes performances.
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Électrophorèse capillaire.
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SCIENCE -- Chemistry -- Organic.
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Capillary electrophoresis
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Carbohydrates -- Analysis
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High performance liquid chromatography
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HPLC.
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Capillaire elektroforese.
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Koolwaterstoffen.
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| Indexed Term |
Carbohydrates Chemical analysis |
| Added Author |
El Rassi, Ziad.
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| Other Form: |
Print version: Carbohydrate analysis. Amsterdam ; New York : Elsevier, 1995 0444899812 9780444899811 (DLC) 95155003 (OCoLC)31697639 |
| ISBN |
9780444899811 |
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0444899812 |
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9780080858661 (electronic bk.) |
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008085866X (electronic bk.) |
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1281779091 |
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9781281779090 |
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9786611779092 |
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6611779094 |
| Standard No. |
AU@ 000056661517 |
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DEBBG BV039828440 |
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DEBBG BV042314096 |
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DEBSZ 407378995 |
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DEBSZ 434136042 |
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DEBSZ 482362197 |
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NZ1 15189031 |
