| Description |
1 online resource (approximately 1 page) : illustrations (chiefly color). |
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text txt rdacontent |
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computer c rdamedia |
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online resource cr rdacarrier |
| Series |
NREL/PO ; 2700-71427 |
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NREL/PO ; 2700-71427.
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| Note |
"40th Symposium on Biotechnology for Fuels and Chemicals Hilton Clearwater Beach Resort & Spa - Clearwater Beach, Florida April 29 - May 2, 2018." |
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Description based on online resource; title from PDF caption (NREL, viewed on Dec. 16, 2021). |
| Summary |
The low secretion levels of cellobiohydrolaseI (CBHI) in yeasts is one of the key barriers preventing yeast from directly degrading and utilizing lignocellulose. To overcome this barrier, we have explored the approach of genetically linking an easily secreted protein to CBHI, with CBHI being the last to be folded. The Trichoderma reesei eg2 (TrEGII) gene was selected as the leading gene due to its previously demonstrated outstanding secretion in yeast. To comprehensively characterize the effects of this fusion protein, we tested this hypothesis in three industrially relevant yeasts: Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi. |
| Subject |
Cellulose 1,4-beta-cellobiosidase.
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Lignocellulose.
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Yeast -- United States.
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Lignocellulose.
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Levure -- États-Unis.
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Cellulose 1,4-beta-cellobiosidase
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Lignocellulose
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Yeast
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United States https://id.oclc.org/worldcat/entity/E39PBJtxgQXMWqmjMjjwXRHgrq
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| Indexed Term |
CBHI |
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cellobiohydrolaseI |
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Lipomyces starkeyi |
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Saccharomyces cerevisiae |
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Yarrowia lipolytica |
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yeasts |
| Genre/Form |
technical reports.
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Technical reports
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Technical reports.
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Rapports techniques.
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| Added Author |
National Renewable Energy Laboratory (U.S.), issuing body.
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| Added Title |
Expression and characterization of chimeric cellobiohydrolase I in yeasts and effects of cellobiohydrolase I fusion to EGII |
| Standard No. |
1500084 OSTI ID |
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0000-0002-5709-914X |
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0000-0002-3550-188X |
| Gpo Item No. |
0430-P-17 (online) |
| Sudoc No. |
E 9.28:NREL/PO-2700-71427 |
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