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Author Porcu, Eleonora.

Title Handbook of Human Oocyte Cryopreservation.

Imprint Cambridge : Cambridge University Press, 2012.


Location Call No. OPAC Message Status
 Axe EBSCO Clinical Nursing E-Book  Electronic Book    ---  Available
Description 1 online resource (160 pages)
text txt rdacontent
computer c rdamedia
online resource cr rdacarrier
Note Print version record.
Contents Chapter 1 Why freeze human eggs? Clinical and biological indications for human oocyte cryopreservation; Ethical and legal indications; Fertility-saving indications; IVF laboratory flexibility indication; The controversial family planning indication; Chapter 2 The evolution of human oocyte cryopreservation; Preliminary research; Early animal studies; Early human studies; Subsequent research; Recent clinical application; Safety of oocyte cryopreservation; Chapter 3 Clinical management of oocyte cryopreservation; Ovarian stimulation and oocyte storage.
Storage of surplus eggs in a standard cycleEgg storage to avoid ovarian hyperstimulation syndrome; Egg storage in polycystic ovary syndrome; Egg storage in cancer patients; Chapter 4 Laboratory management of oocyte cryopreservation; The egg freezing laboratory: location, equipment, instruments, materials; Selecting freezable eggs: the freezable egg score; Evaluation of oocyte maturity and quality; Evaluation of cumulus-corona-oocyte complex maturity; Methodology; Evaluation of the nucleus; Evaluation of the cytoplasm; Cytoplasmic abnormalities; Extracytoplasmic abnormalities.
Zona pellucida (ZP)Perivitelline space (PVS); Perivitelline space granules; First polar body fragmentation; Evaluation of the meiotic spindle; Grading of the oocyte; The freezable egg score; Survived oocyte; Degenerated first polar body; Degenerated oocyte; Oocyte with some sign of degeneration; Steps and timing of the entire cryopreservation procedure; Timing of the events that occur after HCG administration until embryo transfer; Timing of semen sample production; Organizing criteria for storage in liquid nitrogen tanks; Cryobiology; Chapter 5 Slow freezing; Principles.
Evolution of the slow freezing methodologySlow freezing/rapid thawing; Materials; Solutions for slow freezing; Thawing solutions for human oocytes; Setting up for a freezing cycle; Setting up for a thawing cycle; Freezing solutions at different sucrose concentrations; Thawing solutions with differentiated sucrose concentrations; Setting up for a freezing cycle with differential sucrose concentrations; Setting up for a thawing cycle with differential sucrose concentrations; Recipe formula for Na depleted PBS.
Volume, temperature and time of exposure during slow freezing utilizing solutions with 0.1 or 0.2 or 0.3M sucrose at freezing and thawing (freezing 0.1M/thawing 0.1M-freezing 0.2M/thawing 0.2M-freezing 0.3M/thawing 0.3M)Volume, temperature and time of exposure during slow freezing utilizing solutions with differentiated sucrose concentrations (freezing 0.2M sucrose/thawing 0.3M); Starting the freezing cycle; Loading the straw into the programmable freezer; Beginning of the freezing program; Seeding; Storage of the samples; Automated computerized freezing machine; Rapid thawing; Methodology.
Note Volume, temperature and time of exposure during rapid thawing.
Summary An indispensable practical tool for embryologists and reproductive medicine specialists seeking to offer their patients this revolutionary treatment option.
Bibliography Includes bibliographical references and index.
Subject Cryopreservation of organs, tissues, etc.
Oocytin -- Cytology.
Cryopreservation -- methods.
Oocytes -- cytology.
NATURE -- Reference.
SCIENCE -- Life Sciences -- Biology.
SCIENCE -- Life Sciences -- General.
Cryopreservation of organs, tissues, etc. (OCoLC)fst00884506
Genre/Form Electronic books.
Added Author Ciotti, Patrizia.
Venturoli, Stefano.
Other Form: Print version: Porcu, Eleonora. Handbook of Human Oocyte Cryopreservation. Cambridge : Cambridge University Press, 2012 9780521192392
ISBN 9781139841955
9781139839570 (electronic bk.)
1139839578 (electronic bk.)
9780511977879 (electronic bk.)
0511977875 (electronic bk.)
Standard No. DEBSZ 397421419
DEBSZ 431258503

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